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Ubiquitin Antibody


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Our Abpromise guarantee covers the use of ab7780 in the following tested applications.

This image is courtesy of an Abreview submitted by Armen Petrosyan

Inquiry: I have ordered this antibody and I am wondering if this antibody cross-reacts with SUMO proteins since the monoubiquitin-conjugated protein that Im interested in can also be post-translationally modified by sumoylation.

We do not have any experimental data regarding the cross-reactivity of ab7780 with SUMO proteins. I compared the immunogen for ab7780 with the sequences of SUMO 1, 2, 3, 4 (P63165, P61956, P55854, Q6EEV6-Uniprot) and the percentage homology is l...

Thanks for the positive control and technical advise. It WORKED !..... I think the problem was 5% milk solution as blocker and Ab diluent. When I replaced with 3% BSA it worked although BSA did generated high background, I was able to see the ubiquitinate...

I am happy to hear that the antibody is working for you when using BSA instead of milk! If you have any additional questions, please let me know and I will be happy to help you further.

Any products that require an MSDS form will have a link on the products online datasheet to the data. It will be a PDF document link labeled SDS. Please see attached image for example of what to look for on the datasheet. If no SDS link is ...

Thank you for your advises/tips. Should ubiquitin appear as 8 kDa protein or part of the ubiquitinated protein? Please kindly advise me.

Thank you for your reply. This antibody should bind to both the 8.5 kDa free ubiquitin and any other ubiquitinated proteins in your sample. For that reason, you may observe multiple bands in WB. If you are looking to see if one protein in particular ...

I tested the replacement antibody it did not work again. I had some brain lysate as positive control and it did not work. Do you suggest any other positive control that I can use and verify whether Ab is working. Please kindly advise me.

Thank you for contacting us. I am sorry that the replacement antibody is also giving no signal in WB. Ubiquitin should be expressed in all tissues and cell types, but it is possible that the protein is not being effectively released from your samples. I w...

Thank you for your call today and for letting us know about the trouble with this antibody. As we discussed, Im sending a free of charge vial of ab7254 on the order ***, which should arrive tomorrow. Please keep me updated about the r...

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab20137 and one vial of ab131107. Expect this shipment to arri...

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caus...

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